Fiscal Year
2013
Title
The mode of action of neuropeptide in gametogenesis and application to artificial induction of gonad development in bivalves
Project and Theme
Project 1. Study on ecological succession of fisheries ground
Representative Organization: Tohoku University
Theme4. Aquaculture environment in the coastal water of Miyagi Prefecture and innovation of aquaculture system| Representative person | Makoto Osada |
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| Organization | Tohoku University |
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| Department | Graduate School of Agricultural Science |
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Research InformationPeriod and Interval2013/04/01 - 2014/03/31
Research Area
CategoryOthers
OverviewIdentification of neuropeptides controling bivalve gametogenesis from several bivalve species and reproduction-related genes induced by neuropeptide, functionional relationship among them, and the in vivo and in vitro effect of the peptide on gamete formation is demonstrated for artificial induction of gonad development by the neuropeptide.
Overview of the Observation
Research Point Table
| Point | | Name | Konori-hama |
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| Coordinates | 38.437365,141.459654 |
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| Remarks | Station for culture of GnRH-injected scallops |
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Results and Future PlanIn addition to the scallop and oyster, we succeeded to isolate genes encoding prepro-gonadotropin releasing hormone (prepro GnRH) from mussel, surf clam and asian clam and their conserved peptide domains were predicted. In the scallop two type of pyGnRH peptide sequences were determined first. Transcriptome analysis of the mature scallop testis using next generation sequencer provided the several genes related to steroidogenic enzyme, which are supposed to be under the control of GnRH. Moreover, the administration of pyGnRH peptide into scallop promoted the development of testis and induced sex change from female to male, suggesting the capability of artificial induction of maturity and sex manipulation for development of artificial seed production technology.
Investigation Item and Data Acquisition
| Investigation Item | Data Acquisition |
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| Identification of neuropeptides from several bivalves | Primary structure of prepro neuropeptide and prediction of peptide |
| Genes induced by the neuropeptide | Identification of neuropepetide-induced genes by transcriptome analysis and predicted function |
| In vivo and in vitro experiment of the neuropeptide | Morphological development of gonad and germ cell and responsive hormones, proteins and genes |
Reference
Person in charge of Investigation
| Representative person | Makoto Osada |
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| Organization | Tohoku University |
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| Department | Graduate School of Agricultural Science |
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| Fiscal Year | 2013 |
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| Organization | Tohoku University |
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| Category | Others |
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| Research Area | South Sanriku |
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| Discipline | Ocean biology / Ocean ecology -> Physiology
Ocean biology / Ocean ecology -> Mollusks
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