The mode of action of neuroprotein in spawning and application to artificial induction of spawning in bivalves

Fiscal Year

2013

Title

The mode of action of neuroprotein in spawning and application to artificial induction of spawning in bivalves

Project and Theme

Project 1. Study on ecological succession of fisheries ground
Representative Organization: Tohoku University
Theme4. Aquaculture environment in the coastal water of Miyagi Prefecture and innovation of aquaculture system
Representative personMakoto Osada
OrganizationTohoku University
DepartmentGraduate School of Agricultural Science

Research Information

Period and Interval
2013/04/01 - 2014/03/31
Research Area
Onagawa Bay and other experimental waters
Category
Others
Overview
The structure and function of the neuroprotein which suppressively regulates gamete release in bivalves is demonstrated. The relationship between the suppressive neuroprotein and serotonin as an inducer of spawning and the mode of action of the neuroprotein in the release of egg and sperm will be revealed.

Overview of the Observation

Results and Future Plan
To understand the suppressive mechanism of OMAF on the 5-HT (serotonin)-induced spawning the production of reconbinant protein of OMAF is under way using a new expression system. A major function of OMAF for oocyte maturation and activation of sperm motility was demonstrated to be an influx of extracellular Ca2+ by ratioimaging, suggesting that the inhibition of OMAF interfering 5-HT-induced extracellular Ca2+ influx is the most important direction to achieve a complete induction of spawning by 5-HT in bivalve species.

Investigation Item and Data Acquisition

Investigation ItemData Acquisition
Structural of the suppressive neuroproteinFunction of each domain of the neuroprotein
Mode of action of the neuroprotein in final maturation of gameteIntracellular signal transduction involved in final maturation

Reference

Related Observation Plan
FY2013 The mode of action of neuroprotein in spawning and application to artificial induction of spawning in bivalves

Person in charge of Investigation

Representative personMakoto Osada
OrganizationTohoku University
DepartmentGraduate School of Agricultural Science

Keywords

Fiscal Year2013
OrganizationTohoku University
CategoryOthers
Research AreaSouth Sanriku
DisciplineOcean biology / Ocean ecology -> Physiology
Ocean biology / Ocean ecology -> Mollusks