Development of a DNA genotyping method using Next-Generation Sequencing and genome-wide DNA polymorphisms analysis

Fiscal Year

2014

Title

Development of a DNA genotyping method using Next-Generation Sequencing and genome-wide DNA polymorphisms analysis

Project and Theme

Project 1. Study on ecological succession of fisheries ground
Representative Organization: Tohoku University
Theme2. Ecosystems and genetic researches to conserve and restore the coastal fisheries areas in Miyagi Prefecture
Representative personYukio Agatsuma
OrganizationTohoku University
DepartmentGraduate School of Agricultural Science

Research Information

Period and Interval
2014/06/01 - 2015/03/31
Research Area
Sendai Bay, coast lines of Japan
Category
Others
Overview
DNA markers using a huge amount of data produced by a Next Generation Sequencing platform IonTorrent PGM will be newly developped to evaluate genetic diversity of aquatic organisms inhabiting Tohoku area. Based on the spatial and temporal sampling collection, the genetic diversity and its dynamics before and after the impact will be investigated. The species of interest are Pseudopleuronectes yokohamae, Onchoryncus keta, seaweed, and invertebrate animals.

Investigation Item and Data Acquisition

Investigation ItemData Acquisition
Genetic analysisDNA polymorphic information

Reference

Related Observation Result
FY2014 Development of a DNA genotyping method using Next-Generation Sequencing and genome-wide DNA polymorphisms analysis

Person in charge of Investigation

Representative personYuki Minegishi
OrganizationTohoku University
DepartmentGraduate School of Agricultural Science

Contact person about data & samples

Representative personMinoru Ikeda
OrganizationTohoku University
DepartmentLaboratory of Integrative Aquatic Biology, Graduate School of Agricultural Science

Keywords

Fiscal Year2014
OrganizationTohoku University
CategoryOthers
Research AreaIshinomaki Bay
DisciplineOcean biology / Ocean ecology -> Fishes
Ocean biology / Ocean ecology -> Others