Period and Interval2014/04/01 - 2015/03/31
Research Area
Tohoku, Sea of Japan, Okhotsk
CategoryOthers(Genome analysis)
OverviewCoastal marine organisms have been influenced by global climatic changes irrespective of the effect of earthquake and tsunami. To evaluate the effects of 2011.3.11 earthquake on marine organisms, we need examine the distribution and population changes from several decade ago. In this study, we evaluate the effects of both global climate changes and earthquake by analyzing the population changes using Bottom-trawl survey data. In addition, focusing on Gadus macrocephalus, we developed genome-wide SNP markers and detect the markers relating to environmental adaptation, and using these SNP makers, we develop the method evaluating population viability responding to the environmental changes. MethodWe used captured data from 1993 to 2012 for 7 demersal species, and obtained environmental variable (surface and bottom water temperature, salinity, current) for JCOPE. Then, we estimate the CPUE dynamics of these fishes. In this year, we obtained data for Doto, Okhotsk, Sea of Japan, in addition the Tohoku coast. We used GAM analysis to detect the effect of temperature, salinity,water depth and year on CPUE. We conducted RAD-seq analyais for 234 individuals form 19 population around the coast of Japan. We obtained 12942 SNP markers. Among them 389 to 2563 SNP were effective to use population genetic analysis.
Results and Future PlanWe analyzed dynamics of CPUE for 7 species from 1993 to 2012. Most of the species did not dcrease in CPUE after 2011 earthquake except Pseudopleuronectes herzensteini and Hippoglossoides dubius. For G. Macrocephalus, the different responsed to bottom water temperature was found between Tohoku and Sea of Japan. Using 389 SNP among 19 populations, there are different genetc structure among Torror, Sea of Japan, Okhotsk and Tohoku. Especially, in the coast of Tottori where the bottom water temperature is high, it showed distinct genetic structure. Using three different methods, 4 SNP (glucosamily (N-acetly) transferase 3, forming binding protein) were found to be candidate genetic regions that has been subject to selection. We will plan to examine the relationship between allele of SNP and environment variables.