The mode of action of neuropeptide in gametogenesis and application to artificial induction of gonad development in bivalves

Fiscal Year

2015

Title

The mode of action of neuropeptide in gametogenesis and application to artificial induction of gonad development in bivalves

Project and Theme

Project 1. Study on ecological succession of fisheries ground
Representative Organization: Tohoku University
Theme4. Aquaculture environment in the coastal water of Miyagi Prefecture and innovation of aquaculture system
Representative personMakoto Osada
OrganizationTohoku University
DepartmentGraduate School of Agricultural Science

Research Information

Period and Interval
2015/04/01 - 2016/03/31
Research Area
Onagawa Bay and other experimental waters
Category
Others
Overview
We aim to identification of several genes (e.g., neuropeptides, steroidogenic enzymes, which are supposed to be under the control of GnRH and hormones related with reproduction) from several bivalve species by using PCR and in silico cloning with next-generation sequencing. In addition, we plan to carry out the in vivo experiment with the neuropeptides whether they have a potential for controlling scallop gonad maturation in vivo. While, we also plan to produce the recombinant protein for oocyte maturation arresting factor (OMAF) which inhibitory regulates oocyte release in bivalves by using brand-new bacterial expression system.

Investigation Item and Data Acquisition

Investigation ItemData Acquisition
Identification of neuropeptides from several bivalvesPrimary structure of prepro neuropeptide and prediction of peptide
Genes induced by the neuropeptideIdentification of neuropepetide-induced genes by transcriptome analysis and predicted function
In vivo experiment of the neuropeptideMorphological observation of gonad development and responsive hormones, proteins and genes
Production of recombinant protein for oocyte maturation arresting factor (OMAF)Construction of expression vector

Reference

Related Observation Result
FY2015 The mode of action of neuropeptide in gametogenesis and application to artificial induction of gonad development in bivalves

Person in charge of Investigation

Representative personMakoto Osada
OrganizationTohoku University
DepartmentGraduate School of Agricultural Science

Keywords

Fiscal Year2015
OrganizationTohoku University
CategoryOthers
Research AreaSouth Sanriku
DisciplineOcean biology / Ocean ecology -> Physiology
Ocean biology / Ocean ecology -> Mollusks