Researcher Information
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Research Interests
Research activity information
Papers
- Yutetsu Kuruma; Hidetaka Nomaki; Noriyuki Isobe; Daisuke Matsuoka; Yasuhiro Shimane
The Potential of Artificial Cells Functioning under In Situ Deep-Sea Conditions
ACS Synthetic Biology, 01 Oct. 2024 - Rena Matsumoto; Tatsuya Niwa; Yasuhiro Shimane; Yutetsu Kuruma; Hideki Taguchi; Takashi Kanamori
Regulated N-Terminal Modification of Proteins Synthesized Using a Reconstituted Cell-Free Protein Synthesis System.
ACS synthetic biology, 21 Jul. 2023
12, 7, 1935, 1942 - Sumie Eto; Rumie Matsumura; Yasuhiro Shimane; Mai Fujimi; Samuel Berhanu; Takeshi Kasama; Yutetsu Kuruma
Phospholipid synthesis inside phospholipid membrane vesicles.
Communications biology, 27 Sep. 2022
5, 1, 1016, 1016
[Reviewed] - Yasuhiro Shimane; Yutetsu Kuruma
Rapid and Facile Preparation of Giant Vesicles by the Droplet Transfer Method for Artificial Cell Construction.
Frontiers in bioengineering and biotechnology, 2022
10, 873854, 873854
[Reviewed] - Sabrina Galiñanes Reyes; Yutetsu Kuruma; Mai Fujimi; Masako Yamazaki; Sumie Eto; Shota Nishikawa; Satoshi Tamaki; Asaki Kobayashi; Ryo Mizuuchi; Lynn Rothschild; Mark Ditzler; Kosuke Fujishima
PURE mRNA display and cDNA display provide rapid detection of core epitope motif via high‐throughput sequencing
Biotechnology and Bioengineering, Apr. 2021
118, 4, 1702, 1715
[Reviewed] - Tony Z. Jia; Yutetsu Kuruma
Increasing complexity of primitive compartments
Biophysics and Physicobiology, 2021
18, 269, 273 - Tomoya BABA; Shigeyuki KAKIZAWA; Hiroshi MORI; Yutetsu KURUMA; Ken KUROKAWA; Taku OSHIMA
Minimal Genomes: How Many Genes Does a Cell Require To Be Viable
Journal of Geography (Chigaku Zasshi), 25 Dec. 2020
129, 6, 805, 824 - Po-Hsiang Wang; Kosuke Fujishima; Samuel Berhanu; Yutetsu Kuruma; Tony Z. Jia; Anna N. Khusnutdinoya; Alexander F. Yakunin; Shawn E. McGlynn
A Bifunctional Polyphosphate Kinase Driving the Regeneration of Nucleoside Triphosphate and Reconstituted Cell-Free Protein Synthesis
ACS SYNTHETIC BIOLOGY, Jan. 2020
9, 1, 36, 42
[Reviewed] - Tony Z. Jia; Yutetsu Kuruma
Recent Advances in Origins of Life Research by Biophysicists in Japan
Challenges, 11 Apr. 2019
10, 1, 28, 28 - Berhanu S; Ueda T; Kuruma Y
Artificial photosynthetic cell producing energy for protein synthesis.
Nature communications, Mar. 2019
10, 1, 1325
[Reviewed] - Sawasato K; Sato R; Nishikawa H; Iimura N; Kamemoto Y; Fujikawa K; Yamaguchi T; Kuruma Y; Tamura Y; Endo T; Ueda T; Shimamoto K; Nishiyama KI
CdsA is involved in biosynthesis of glycolipid MPIase essential for membrane protein integration in vivo.
Scientific reports, Feb. 2019
9, 1, 1372
[Reviewed] - Sasaki M; Nishikawa H; Suzuki S; Moser M; Huber M; Sawasato K; Matsubayashi H; Kumazaki K; Tsukazaki T; Kuruma Y; Nureki O; Ueda T; Nishiyama K
The bacterial protein YidC accelerates MPIase-dependent integration of membrane proteins
J Biol Chem, 2019
294, 49, 18898, 18908
[Reviewed] - Pasquale Stano; Yutetsu Kuruma; Luisa Damiano
Synthetic biology and (embodied) artificial intelligence: opportunities and challenges
Adaptive Behavior, 01 Feb. 2018
26, 1, 41, 44
[Reviewed] - Luisa Damiano; Yutetsu Kuruma; Pasquale Stano
Synthetic biology and artificial intelligence: toward cross-fertilization
Complex Systems, 2018
27, 3, i, vii - Giordano Rampioni; Francesca D'Angelo; Marco Messina; Alessandro Zennaro; Yutetsu Kuruma; Daniela Tofani; Livia Leoni; Pasquale Stano
Synthetic cells produce a quorum sensing chemical signal perceived by: Pseudomonas aeruginosa
Chemical Communications, 2018
54, 17, 2090, 2093
[Reviewed] - Furusato, Takumi; Horie, Fumihiro; Matsubayashi, Hideaki T.; Amikura, Kazuaki; Kuruma, Yutetsu; Ueda, Takuya
De Novo Synthesis of Basal Bacterial Cell Division Proteins FtsZ, FtsA, and ZipA Inside Giant Vesicles
ACS Synthetic Biology, 2018
7, 4, 953, 961
[Reviewed] - Kanamori, Takashi; Nagaike, Takashi; Kuruma, Yutetsu; Ueda, Takuya; Corporation, Genefrontier; Plaza, Todai-kashiwa Venture
Cell-free translation system: Development in biochemistry and advance in synthetic biology
Journal of Japanese Biochemical Society, 2017
89, 2, 211, 220
[Reviewed] - Luisa Damiano; Yutetsu Kuruma; Pasquale Stano
What can synthetic biology offer to artificial intelligence (and vice versa)?
BIOSYSTEMS, Oct. 2016
148, 1, 3
[Reviewed] - Yutetsu Kuruma; Takuya Ueda
The PURE system for the cell-free synthesis of membrane proteins (vol 10, pg 1328, 2015)
NATURE PROTOCOLS, Mar. 2016
11, 3, 616, 616
[Reviewed] - Yutetsu Kuruma
Creation of Simple Biochemical Systems to Study Early Cellular Life
ORIGINS OF LIFE AND EVOLUTION OF BIOSPHERES, Sep. 2015
45, 3, 359, 360
[Reviewed] - Yutetsu Kuruma; Takuya Ueda
The PURE system for the cell-free synthesis of membrane proteins
NATURE PROTOCOLS, Sep. 2015
10, 9, 1328, 1344
[Reviewed] - Hideaki Matsubayashi; Yutetsu Kuruma; Takuya Ueda
Cell-Free Synthesis of SecYEG Translocon as the Fundamental Protein Transport Machinery
ORIGINS OF LIFE AND EVOLUTION OF BIOSPHERES, Dec. 2014
44, 4, 331, 334
[Reviewed] - Pier Luigi Luisi; Yutetsu Kuruma
Open Questions on the Origin of Life (OQOL)-Introduction to the Special Issue
ORIGINS OF LIFE AND EVOLUTION OF BIOSPHERES, Dec. 2014
44, 4, 267, 268
[Reviewed] - Hideaki Matsubayashi; Yutetsu Kuruma; Takuya Ueda
In Vitro Synthesis of the E. coli Sec Translocon from DNA
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, Jul. 2014
53, 29, 7535, 7538
[Reviewed] - Yutetsu Kuruma; Hideaki Matsubayashi; Takuya Ueda
In vitro reconstruction of functional membrane
Artificial Life 14 - Proceedings of the 14th International Conference on the Synthesis and Simulation of Living Systems, ALIFE 2014, 2014
963, 964 - Matsubayashi Hideaki; Kuruma Yutetsu; Ueda Takuya
1P222 In vitro synthesis of SecYEG translocon(13D. Biological & Artificial membrane: Transport,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))
Seibutsu Butsuri, 2014
54, 1, S177 - Yoshihiro Shimizu; Yutetsu Kuruma; Takashi Kanamori; Takuya Ueda
The PURE system for protein production
Methods in Molecular Biology, 2014
1118, 275, 284
[Reviewed] - Pauline van Nies; Zohreh Nourian; Maurits Kok; Roeland van Wijk; Jonne Moeskops; Ilja Westerlaken; Jos M. Poolman; Rienk Eelkema; Jan H. van Esch; Yutetsu Kuruma; Takuya Ueda; Christophe Danelon
Unbiased Tracking of the Progression of mRNA and Protein Synthesis in Bulk and in Liposome-Confined Reactions
CHEMBIOCHEM, Oct. 2013
14, 15, 1963, 1966
[Reviewed] - Yutetsu Kuruma; Hideaki Matsubayashi
Autonomous construction of synthetic cell membrane
Advances in Artificial Life, ECAL 2013, 02 Sep. 2013
9, 10 - Pasquale Stano; Tereza Pereira de Souza; Yutetsu Kuruma; Paolo Carrara; Pier Luigi Luisi
Semi-synthetic minimal cells: Biochemical, physical, and technological aspects
Synthetic Biology, 2013
261, 276
[Reviewed] - Yutetsu Kuruma; Toshiharu Suzuki; Sakurako Ono; Masasuke Yoshida; Takuya Ueda
Functional analysis of membranous F-0-a subunit of F1F0-ATP synthase by in vitro protein synthesis
BIOCHEMICAL JOURNAL, Mar. 2012
442, 3, 631, 638
[Reviewed] - Pasquale Stano; Tereza Pereira De Souza; Matteo Allegretti; Yutetsu Kuruma; Pier Luigi Luisi
New and unexpected insights on the formation of protocells from a synthetic biology approach: The case of entrapment of biomacromolecules and protein synthesis inside vesicles
The Minimal Cell: The Biophysics of Cell Compartment and the Origin of Cell Functionality, 2011
195, 216
[Reviewed] - Pasquale Stano; Paolo Carrara; Yutetsu Kuruma; Tereza Pereira de Souza; Pier Luigi Luisi
Compartmentalized reactions as a case of soft-matter biotechnology: synthesis of proteins and nucleic acids inside lipid vesicles
JOURNAL OF MATERIALS CHEMISTRY, 2011
21, 47, 18887, 18902
[Reviewed] - Yutetsu Kuruma; Toshiharu Suzuki; Masasuke Yoshida; Takuya Ueda
Artificial organelle for energy production in artificial cell.
Advances in Artificial Life: 20th Anniversary Edition - Back to the Origins of Alife, ECAL 2011, Paris, France, August 8-12, 2011, 2011
438
[Reviewed] - Yutetsu Kuruma; Suzuki Toshiharu; Yoshida Masasuke; Ueda Takuya
1P247 1I1520 Synthesis of Functional Biomembrane by Cell-Free Protein Productions(Biol & Artifi memb.:Dynamics,Oral Presentations,The 48th Annual Meeting of the Biophysical Society of Japan)
Seibutsu Butsuri, 2010
50, 2, S63 - Stano P; Kuruma Y; Souza TP; Luisi PL
Biosynthesis of proteins inside liposomes.
Methods in molecular biology (Clifton, N.J.), 2010
606, 127, 145
[Reviewed] - Kuruma Y; Suzuki T; Ueda T
Production of multi-subunit complexes on liposome through an E. coli cell-free expression system.
Methods in molecular biology (Clifton, N.J.), 2010
607, 161, 171
[Reviewed] - Yutetsu Kuruma; Pasquale Stano; Takuya Ueda; Pier Luigi Luisi
A synthetic biology approach to the construction of membrane proteins in semi-synthetic minimal cells
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, Feb. 2009
1788, 2, 567, 574
[Reviewed] - Kuruma Y; Stano P; Ueda T; Luisi PL
A synthetic biology approach to the construction of membrane proteins in semi-synthetic minimal cells.
Biochimica et biophysica acta, Feb. 2009
1788, 2, 567, 574
[Reviewed] - Yoko Ozaki; Toshiharu Suzuki; Yutetsu Kuruma; Takuya Ueda; Masasuke Yoshida
UncI protein can mediate ring-assembly of c-subunits of FoF1-ATP synthase in vitro
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Mar. 2008
367, 3, 663, 666
[Reviewed] - Kuruma Yutetsu; Stano Pasquale; Ueda Takuya; Luisi Pier Luigi
2P-239 Cell-free expression of phospholipid-synthesizing membrane proteins in the interior of liposomes(The 46th Annual Meeting of the Biophysical Society of Japan)
Seibutsu Butsuri, 2008
48, S112 - Giovanni Murtas; Yutetsu Kuruma; Paolo Bianchini; Alberto Diaspro; Pier Luigi Luisi
Protein synthesis in liposomes with a minimal set of enzymes
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Nov. 2007
363, 1, 12, 17
[Reviewed] - Yutetsu Kuruma
Biosynthesis of phosphatidic acid in liposome compartments - Toward the self-reproduction of minimal cells
ORIGINS OF LIFE AND EVOLUTION OF BIOSPHERES, Oct. 2007
37, 4-5, 409, 413
[Reviewed] - Yoshihiro Shimizu; Yutetsu Kuruma; Bei-Wen Ying; So Umekage; Takuya Ueda
Cell-free translation systems for protein engineering
FEBS JOURNAL, Sep. 2006
273, 18, 4133, 4140
[Reviewed] - Y Kuruma; K Nishiyama; Y Shimizu; M Muller; T Ueda
Development of a minimal cell-free translation system for the synthesis of presecretory and integral membrane proteins
BIOTECHNOLOGY PROGRESS, Jul. 2005
21, 4, 1243, 1251
[Reviewed]
Books and Other Publications
Presentations
- 田中 大貴, 松村 るみゑ, 嶋根 康弘, 車 兪てつ
NADH を自己生産してリン脂質を合成する人工細胞
無細胞生命科学研究会, 15 Mar. 2024 - 車 兪てつ, 江藤 澄江, 松村 るみゑ, 嶋根 康弘, 藤見 麻衣, BERHANU Samuel, 笠間 健嗣
膜内反応場で脂質を合成する人工細胞
日本分子生物学会, 06 Dec. 2023 - 車 兪徹
膜内反応場で脂質を合成する人工細胞
第46回日本分子生物学会年会, 06 Dec. 2023, [Invited] - 車 兪てつ、江藤 澄江、松村 るみゑ、嶋根 康弘、ベルハヌ サミュエル、笠間 健嗣、藤見 麻衣
複数のサブシステムから構成される人工細胞
日本生物物理学会, 15 Nov. 2023 - Yutetsu Kuruma
Functional expression in artificial cell system composed of multi-subsystems
第61回日本生物物理学会年会, 15 Nov. 2023, [Invited] - 松本 令奈、丹羽 達也、嶋根 康弘、車兪 てつ、田口 英樹、金森 崇
再構成型無細胞タンパク質合成系(PUREfrex®)を用いた標的タンパク質の N 末端翻訳後修飾
「細胞を創る」研究会, 25 Sep. 2023 - 江藤澄江、松村るみゑ、嶋根康弘、藤見麻衣、サミュエル・ベルハヌ、笠間健嗣、車兪てつ
リン脂質の膜の中でリン脂質を合成する人工細胞
「細胞を創る」研究会, 25 Sep. 2023 - 車 兪徹
膜にフォーカスした人工細胞研究
第63回生物物理若手の会夏の学校, 06 Sep. 2023, [Invited] - 車 兪徹
人工細胞構築のストラテジーとその挑戦
理化学研究所 Synthetic Biology -生物学の新たな潮流-, 02 Aug. 2023, [Invited] - Yutetsu Kuruma
Construction of autopoietic artificial cell -toward construction of self-reproducing man-made cells-
ALIFE 2023, 25 Jul. 2023, [Invited] - 車兪徹, 江藤澄江, 松村るみゑ, 嶋根康弘, 藤見麻衣, ベルハヌ サミュエル, 笠間健嗣
人工細胞システムの更なる高度化・複雑化と応用
日本蛋白質科学会, 06 Jul. 2023 - 車 兪徹
人工細胞システムの更なる高度化・複雑化と応用
第23回日本蛋白質科学会年会, 06 Jul. 2023, [Invited] - 松本令奈 , 丹羽達也, 嶋根康弘, 車兪徹, 田口英樹, 金森崇
再構成型無細胞タンパク質合成系(PUREfrex)を用いた標的タンパク質のN末端翻訳後修飾
日本蛋白質科学会, 05 Jul. 2023 - 嶋根康弘、Yutetsu Kuruma
巨大膜脂質小胞の迅速かつ簡単な調製方法
「細胞を創る」研究会15.0, 17 Oct. 2022 - Yutetsu Kuruma
Lipid synthesis in artificial cell
第60回日本生物物理学会年会, 28 Sep. 2022, [Invited] - Yutetsu Kuruma、Yasuhiro Shimane、Rumie Matsumura
脂質を合成する人工細胞／Lipid synthesis in artificial cell
日本生物物理学会シンポジウム, 28 Sep. 2022, [Invited] - Yutetsu Kuruma
Lipid synthesis inside membrane vesicles toward the construction of self-propagating artificial cells
21th HFSP Awardee meeting, 29 Aug. 2022 - 車 兪徹
分子と遺伝子からビルドアップする人工細胞研究
第38回緑陰セミナー, 09 Jul. 2022, [Invited] - 車 兪徹
複数構成因子からなる細胞機能・構造解析のための人工細胞システムの構築
第２２回タンパク質科学会年会, 09 Jun. 2022 - Yutetsu Kuruma
Construction of autotrophic artificial cell by assembling molecules and genes
Pacifichem2021, 19 Dec. 2021, [Invited] - 車 兪徹
合成生物学からみた生命の起源と細胞再構築のための基盤技術
第２３回比較グライコーム研究会, 21 Aug. 2021, [Invited] - 車 兪徹
合成生物学からみた生命の起源と細胞再構築のための基盤技術
第２３回比較グライコーム研究会, 21 Aug. 2021, [Invited] - Yutetsu Kuruma
Bottom-up construction of artificial photosynthetic cell
第43回日本分子生物学会年会 シンポジウム「統合合成生物学：人工細胞モデルから多細胞システム制御まで」, 04 Dec. 2020, [Invited] - kuruma
Construction of artificial photosynthetic cell toward an autonomous system
International Workshop "From Soft Matter to Protocell 2020”, 29 Oct. 2020, [Invited] - Kuruma Yutetsu
人工細胞研究から考える初期生命の様相
第42回日本分子生物学会年会, 03 Dec. 2019, [Invited] - Yutetsu Kuruma
Reconstruction of cellular functions in compartment
ELSI-Mini Symposium on primitive compartmentalization, 18 Nov. 2019, [Invited] - Yutetsu Kuruma
Session Topic: Synthetic Biology Challenges Toward Creating Man-Made Cells
3rd UK-Japan Frontiers of Science Symposium (Fos UK-Jap)（千葉）, 09 Nov. 2019, [Invited] - Kuruma Yutetsu
（もう後には引けない）人工細胞研究
第８回Yet Another Artificial Cell (YAC) 研究会 2019, 07 Nov. 2019, [Invited] - Kuruma Yutetsu
（原理的には）100%定量解析を可能にする人工細胞研究
定量生物学の会北海道キャラバン2019（北大）, 06 Nov. 2019, [Invited] - Kuruma Yutetsu
光依存的にエネルギーを生産する人工細胞の構築
日本表面真空学会学術講演会（つくば）, 30 Oct. 2019, [Invited] - Yutetsu Kuruma
Artificial photosynthetic cell producing energy for protein synthesis
Asian Synthetic Biology Association (ASBA) Meeting （成都、中国）, 27 Oct. 2019, [Invited] - Yutetsu Kuruma
Artificial photosynthetic cell producing energy for protein synthesis
Cold Spring Harbor Asia - Synthetic Biology （蘇州、中国）, 21 Oct. 2019, [Invited] - 車 兪徹、サミュエル ベルハヌ, 上田 卓也
光エネルギーからタンパク質を合成し且つ自分のパーツも作る人工細胞
第12回 「細胞を創る」研究会12.0, 17 Oct. 2019, [Invited] - Yutetsu Kuruma, Samuel Berhanu, Takuya Ueda
光からエネルギーを合成しタンパク質を合成する人工細胞の構築
第５７回日本生物物理学会年会, 24 Sep. 2019, [Invited] - Yutetsu Kuruma, Samuel Berhanu, Takuya Ueda
光からエネルギーを合成しタンパク質を合成する人工細胞の構築
第２９回日本生化学会大会, 18 Sep. 2019, [Invited] - Yutetsu Kuruma
Artificial Photosynthetic Cell
Formal methods for the synthesis of biomolecular circuits, 02 Sep. 2019, [Invited] - Kuruma Yutetsu
細胞を創って考える生命の起源
生命の起源および進化・アストロバイオロジー夏の学校2019, 30 Aug. 2019, [Invited] - Yutetsu Kuruma
Study of the Origin of Life and Artificial Cell
Connecting Fundamental Physics, Chemistry and the Origins of Biomolecular Homochirality, 26 Jun. 2019, [Invited]
