Fiscal Year
2014
Title
The mode of action of neuropeptide in gametogenesis and application to artificial induction of gonad development in bivalves
Project and Theme
Project 1. Study on ecological succession of fisheries ground
Representative Organization: Tohoku University
Theme4. Aquaculture environment in the coastal water of Miyagi Prefecture and innovation of aquaculture system| Representative person | Makoto Osada |
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| Organization | Tohoku University |
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| Department | Graduate School of Agricultural Science |
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Research InformationPeriod and Interval2014/04/01 - 2015/03/31
Research Area
Onagawa Bay and other experimental waters
CategoryOthers
OverviewWe performed molecular identification of several genes (e.g., neuropeptides, steroidogenic enzymes, which are supposed to be under the control of GnRH and hormones related with reproduction) from several bivalve species by using PCR and in silico cloning with next-generation sequencing. In addition, in vivo experiment with the neuropeptides suggested that neuropeptide administration may have a potential for controlling scallop gonad maturation in vivo.While, we started recombinant production for oocyte maturation arresting factor (OMAF) which inhibitory regulates oocyte release in bivalves by using brand-new bacterial expression system.
Overview of the Observation
Research Point Table
| Point | | Name | Konori Bay |
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| Coordinates | 38.437365,141.459654 |
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| Remarks | Rearing area of the py-GnRH-administrated scallops |
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Research Point Map & Track Chart Map & Point List
Results and Future PlanIn addition to the five bivalve species (i.e., scallop, oyster, mussel, surf clam and Asian clam), we hereby cloned the genes encoding gonadotropin releasing hormone (GnRH) from other two bivalves (e.g., Manila clam and bloody clam) and estimated their mature peptide sequences. Full-length cDNAs of several steroidogenic enzyme genes were cloned from a scallop based on the contig sequences generated by the transcriptome data of next-generation sequencing. In addition, the administration of two pyGnRH peptides into scallop promoted the development of testis and inhibited oocyte growth in ovary, suggesting the possibility of artificial induction of sexual maturity and sex manipulation in scallops.
Meanwhile, to understand the mechanism of OMAF on the spawning, the production of recombinant protein of OMAF is now under way. In the future, we plan to use it for in vivo and in vitro experiments with various bivalve species in order to develop an effective method for seed production in marine bivalves.
Investigation Item and Data Acquisition
| Investigation Item | Data Acquisition |
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| Identification of neuropeptides from several bivalves | Primary structure of prepro neuropeptide and prediction of mature peptide sequence |
| Genes induced by the neuropeptide sinaling | Identification of neuropepetide-induced genes by transcriptome analysis and predicted function |
| In vivo experiment of the neuropeptide | Morphological observation of gonad development and responsive hormones, proteins and genes |
| Production of recombinant protein for oocyte maturation arresting factor (OMAF) | Construction of expression vector |
Reference
Person in charge of Investigation
| Representative person | Makoto Osada |
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| Organization | Tohoku University |
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| Department | Graduate School of Agricultural Science |
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| Fiscal Year | 2014 |
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| Organization | Tohoku University |
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| Category | Others |
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| Research Area | South Sanriku |
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| Discipline | Ocean biology / Ocean ecology -> Physiology
Ocean biology / Ocean ecology -> Mollusks
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